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. 2018 Nov 7;38(45):9618–9634. doi: 10.1523/JNEUROSCI.0914-18.2018

Figure 6.

Figure 6.

Age-dependent mRNA expression changes in cortex-derived ECs following CCI injury. ECs were isolated from the cortex of 4 d sham and injured adult and juvenile mice using magnetic bead separation. a, Expression of cell-specific genes was quantified using qRT-PCR and is represented relative to tie2 for each group. PDGFRβ, GFAP, NeuN, and cx3cr1 were absent or reduced compared with tie2 or VE-cadherin in all four groups, indicating EC enrichment following cortex isolation. b, Additional genes were analyzed and represented relative to adult sham. Occludin-1 was significantly increased in adult and juvenile ECs after CCI injury (*p = 0.0217 and ***p = 0.002, respectively); however, juvenile mice showed higher levels compared with adult (*p = 0.0017). Claudin-5 was significantly increased in juvenile injured ECs compared with sham (***p = 0.0006) and adult injured cells (***p = 0.0055). Ang1 and Ang2 were increased only in juvenile injured ECs compared with juvenile sham (**p = 0.0052 and p = 0.0223, respectively). c, Vegf and its receptors, vegfr1 and vegfr2, were significantly reduced in juvenile injured ECs compared with adult injured cells (*p = 0.0232, **p = 0.0093, and *p = 0.0219, respectively). d, No difference in NF-κβ was found; however, vcam and eNOS were significantly reduced in juvenile injured ECs compared with adult injured cells (****p = 0.00001 compared to respective sham levels).