Figure 4.

cbe1 mutant of A. thaliana. A, site of T-DNA insertion in the sixth exon of the CBE1 coding region. The eIF4E-binding motif is present in the second exon. B, RT-PCR of 7-day-old WT and cbe1 seedlings grown on MS plates. CBE1 expression is lost in the cbe1 mutant. TUB2 expression serves as a control. C, cbe1 mutants show developmental delay relative to WT Col0 plants. Six-week-old plants grown under long day conditions (16 h light, 8 h dark) shown. D, cap-binding complex mutants show delayed flowering time under long day conditions. Error bars represent S.E. (n = 24–28). Asterisks indicate significantly delayed flowering time in mutant lines compared with Col0 (p value < 0.05, Student's t test).