Figure 5.

cbe1 mutants show dysregulation of cell cycle gene expression. A, GO term enrichment of cbe1 mutant up-regulated genes by AmiGO 2 (44). No enriched GO terms were observed for down-regulated genes in the mutant. B, qRT-PCR of G₂/M phase–specific genes identified by sequencing as overexpressed in cbe1 plants. Gene expression relative to TUB2 was measured in three biological replicates with at least three technical replicates. Error bars represent S.E. (n = 3–6). Asterisks indicate genes with expression significantly varying in the cbe1 mutant compared with Col0 (p value < 0.05, Student's t test). C, Venn diagram showing overlap of up-regulated genes in cbe1 mutant and G₂/M phase–specific genes (30).