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. 2018 Sep 18;293(44):17154–17165. doi: 10.1074/jbc.RA118.003055

Figure 2.

Figure 2.

lncRNA GAS8-AS1 or GAS8 inhibits cell proliferation of HepG2 or SMMC7721 cells. A and B, overexpression of GAS8-AS1 or GAS8 inhibits HepG2 cell growth. On the contrary, silencing expression of GAS8-AS1 or GAS8 with their siRNAs (siGAS8-AS1-1/-2/-3 or siGAS8-1/-2/-3) significantly accelerates proliferation of HepG2 cells. Cell number was counted at 24, 48, and 72 h after transfection. C and D, similar results were observed in SMMC7721 cells. E, endogenous GAS8-AS1 expression was first silenced using various siRNAs (siGAS8-AS1–1/-2), and GAS8 was then overexpressed using its expression construct in HepG2 cells. Interestingly, GAS8 overexpression could reverse the growth acceleration phenotype induced by GAS8-AS1 silencing. F, colony formation assays indicate that both genes significantly suppress colony formation of both HCC cell lines. At the 12th day after transfection, colony number in each well was counted. All results of the mean of triplicate assays with S.D. (error bars) are presented. *, p < 0.05; **, p < 0.01.