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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

Figure 1.

Figure 1.

Binding of H. pylori to total nonacid glycosphingolipids of human stomach. A–D, thin-layer chromatogram detected with anisaldehyde (A) and autoradiograms obtained by binding of H. pylori strain J99 (B), H. pylori strain J99/BabA− (C), and H. pylori strain 26695 (D). The glycosphingolipids were separated on aluminum-backed silica gel plates, using chloroform/methanol/water 60:35:8 (by volume) as the solvent system, and the binding assays were performed as described under “Experimental procedures.” Autoradiography was for 12 h. Lane 1, total nonacid glycosphingolipids of human stomach blood group O(Rh-)P, 40 μg; lane 2, total nonacid glycosphingolipids of human stomach blood group A(Rh+)P, 40 μg; lane 3, total nonacid glycosphingolipids of human stomach blood group A(Rh+)p, 40 μg; lane 4, reference total nonacid glycosphingolipids of human meconium, 40 μg. The Roman numerals to the left of the chromatogram in A denote the number of carbohydrate unit(s) in the bands.