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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

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© 2018 Jin et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 10. — LC-ESI/MS of the oligosaccharides obtained from the total nonacid glycosphingolipid fractions from human gastric mucosa by hydrolysis with endoglycoceramidase II from Rhodococcus spp. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides obtained from human from human gastric mucosa blood group A(Rh+)P (case 3). Peaks marked with an asterisk are noncarbohydrate contaminants. B, base peak chromatogram from LC-ESI/MS of the oligosaccharides obtained from human from human gastric mucosa blood group A(Rh+)P (case 4). C, molecular ion profile from LC-ESI/MS of from human gastric mucosa blood group A(Rh+)P (case 3). Peaks marked with an asterisk are noncarbohydrate contaminants. D, molecular ion profile from LC-ESI/MS of from human gastric mucosa blood group A(Rh+)P (case 4). The identification of oligosaccharides was based on their retention times, determined molecular masses, and subsequent MS² sequencing. The oligosaccharides identified in the chromatograms were Fucα2Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (Le^b-6), Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^x-5), GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glc (A6-2), Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (Le^a-5), Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^y-6), Galβ4GlcNAcβ3Galβ4Glc (nLc4), GalNAcβ3Galβ4GlcNAcβ3Galβ4Glc (x₂), Fucα2Galβ4GlcNAcβ3Galβ4Glc (H5-2), Fucα2Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (H7-2), Galβ3GlcNAcβ3Galβ4Glc (Lc4), GalNAcα3(Fucα2)Galβ3/4(Fucα4/3)GlcNAcβ3Galβ4Glc (A7), Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (nLc6), and GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (A8).