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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

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© 2018 Jin et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — LC-ESI/MS of the oligosaccharides obtained from the total nonacid glycosphingolipid fraction of human blood group O(Rh−)P stomach by hydrolysis with endoglycoceramidase II from Rhodococcus spp. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides obtained from human stomach blood group O(Rh−)P. B, MS² of the ion at m/z 852 (retention time 15.5 min). C, MS² of the ion at m/z 852 (retention time 15.9 min). D, MS² of the ion at m/z 852 (retention time 20.1 min). E, interpretation formulas showing the deduced oligosaccharide sequences. The identification of oligosaccharides was based on their retention times, determined molecular masses, and subsequent MS² sequencing. The oligosaccharides identified in the chromatograms were Galα4Galβ4Glc (Gb3), GalNAcβ3Galα4Galβ4Glc (Gb4), Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^x-5), Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (Le^a-5), Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^y-6), Galβ4GlcNAcβ3Galβ4Glc (nLc4); Fucα2Galβ4GlcNAcβ3Galβ4Glc (H5-2), GalNAcβ3Galβ4GlcNAcβ3Galβ4Glc (x₂), Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (nLc6), and Fucα2Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (H7-2).