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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

Figure 5.

Figure 5.

LC-ESI/MS of the oligosaccharides obtained by digestion of the slow-migrating nonacid glycosphingolipids of human blood group A(Rh+)P and A(Rh+)p stomachs with Rhodococcus endoglycoceramidase II. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction P-III. B, base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction p-III. C, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from fraction P-III. D, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from fraction p-III. E, MS2 of the ion at m/z 706 (retention time 11.6 min) from LC-ESI/MS of the oligosaccharides derived from fraction P-III. F, MS2 of the ion at m/z 706 (retention time 17.2 min) from LC-ESI/MS of the oligosaccharides derived from fraction P-III. G, MS2 of the ion at m/z 706 (retention time 17.5 min) from LC-ESI/MS of the oligosaccharides derived from fraction P-III. H, interpretation formulas showing the deduced oligosaccharide sequences. The identification of oligosaccharides was based on their retention times, determined molecular masses, and subsequent MS2 sequencing. The oligosaccharides identified in the chromatograms were Galα4Galβ4Glc (Gb3), Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Lex-5), GlcNAcβ3Galβ4Glc (Lc3), GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glc (A6-2), and Fucα2Galβ4GlcNAcβ3Galβ4Glc (H5-2).