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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

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© 2018 Jin et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — LC-ESI/MS of the oligosaccharides obtained by Rhodococcus endoglycoceramidase II digestion of the slow-migrating nonacid glycosphingolipid fraction P-IV from human blood group A(Rh+)P stomach and of reference blood group A heptaosylceramides. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction P-IV. B, MS² of the ion at m/z 1055 (retention time 13.1 min). C, MS² of the ion at m/z 1201 (retention time 11.6 min). D, MS² of the ion at m/z 1201 (retention time 13.8 min). E, MS² of the ion at m/z 1420 (retention time 19.1 min). F, MS² of the ion at m/z 1201 from LC-ESI/MS of the oligosaccharides derived from reference blood group A type 2/ALe^y heptaosylceramide (GalNAcα3(Fucα2)Galβ4(Fucα3)GlcNAcβ3Galβ4Glc). G, MS² of the ion at m/z 1201 from LC-ESI/MS of the oligosaccharides derived from reference blood group A type 1/ALe^b heptaosylceramide (GalNAcα3(Fucα2)Galβ3(Fucα43)GlcNAcβ3Galβ4Glc). H, interpretation formulas showing the deduced oligosaccharide sequences. The identification of oligosaccharides was based on their retention times, determined molecular masses, and subsequent MS² sequencing. The oligosaccharides identified in the chromatograms were GalNAcβ3Galα4Galβ4Glc (Gb4), GalNAcα3(Fucα2)Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (A7-2), Fucα2Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (Le^b-6), Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^x-5), GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glc (A6-2), Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (Le^a-5), GalNAcα3(Fucα2)Galβ3(Fucα4)GlcNAcβ3Galβ4Glc (A7-1), Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^y-6), Fucα2Galβ3GlcNAcβ3Galβ4Glc (H5-1), Galβ4GlcNAcβ3Galβ4Glc (nLc4), GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (A8-2), GalNAcβ3Galβ4GlcNAcβ3Galβ4Glc (x₂), Fucα2Galβ4GlcNAcβ3Galβ4Glc (H5-2), Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (nLc6), and Fucα2Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (H7-2). *, nonoligosaccharide contaminant.