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. 2018 Sep 19;293(44):17248–17266. doi: 10.1074/jbc.RA118.004854

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© 2018 Jin et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 8. — LC-ESI/MS (m/z 1300–2000) of the complex oligosaccharides obtained by digestion of the slow-migrating nonacid glycosphingolipid fraction p-IV-f from human blood group A(Rh+)p stomach with Rhodococcus endoglycoceramidase II. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction p-IV-f. B, MS² of the ion at m/z 1363 (retention time 14.9 min). C, MS² of the ion at m/z 1363 (retention time 19.8 min). D, MS² of the ion at m/z 1509 (retention time 16.5 min). E, MS² of the ion at m/z 1420 (retention time 19.4 min). F, interpretation formulas showing the deduced oligosaccharide sequences. The identification of oligosaccharides was based on their retention times, determined molecular masses, and subsequent MS² sequencing. The oligosaccharides identified in the chromatograms were Fucα2Galβ3/4GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Fuc-Hex-HexNAc-Le^x-5), Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glc (Le^y-9), GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (A8-2), Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (Le^y-8), Fucα2Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (H9-2), and Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glc (nLc8). *, nonoligosaccharide contaminants.