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Dkobs of InhA WT and Y158F
The Dkobs was measured by quantifying the discrimination between H and D addition at enoyl-CoA concentrations that were 20-fold below Km to keep the commitment factor low and a NADH concentration of 10 mm, more than 100-fold over Km, to avoid a change in the commitment factor because of a change in NADH concentration.
| Substrate | InhA WT | InhA Y158F |
|---|---|---|
| Hexenoyl-CoA | 1.74 ± 0.14 | 3.9 ± 0.3 |
| Octenoyl-CoA | 1.28 ± 0.04 | 3.4 ± 0.1 |
| Dodecenoyl-CoA | 1.14 ± 0.03 | 2.4 ± 0.1 |
