Figure 6.

Extension of the CsuC G₁ donor strand motif inhibits CsuA/B polymerization. A, Western blotting of periplasmic extracts of recombinant E. coli co-expressing CsuA/B and CsuC (wildtype (WT) and insertion mutants (INI, INL, LNI)). Complexes were incubated at 22 or 100 °C prior to electrophoresis. NC, negative control: periplasmic extract of noninduced cells; M, marker proteins, molecular weights are indicated. B, ratio of integrated optical density (IOD) of the first band (CsuA/B dimer) to the combined IOD of bands of larger CsuA/B polymers. Error bars indicate S.D. The results are representative of three independent experiments.