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. 2018 Sep 12;293(44):17119–17134. doi: 10.1074/jbc.RA118.003608

Figure 3.

Figure 3.

TEAD4-mediated regulation of adipogenesis is independent of YAP. A and B, Oil Red O staining of lentivirus-mediated overexpression of pLEX/TEAD4 WT/TEAD4 Y429H/TEAD4 mNLS 3T3-L1 stable cell lines after adipogenic mixture induction for 7 days (A) and quantification by measurement of the absorbance at 510 nm (B). ns, not significant. *, p < 0.05 by Student's t test. C, immunoblot (IB) analysis of HA, PPARγ, C/EBPα, and FABP4 expression in the above cell samples. GAPDH was used as an internal control. The positions of protein molecular mass marker are indicated on the right. MW, molecular weight. D, immunofluorescence staining of transfected HA-TEAD4 and HA-TEAD4 mNLS with the indicated antibodies in 293T cells. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). E, effect of ectopic expression of TEAD4 WT, TEAD4 Y429H, and TEAD4 mNLS on PPARγ-Luc activity in 293T cells. Data are shown as mean ± S.D. (n = 3). ***, p < 0.001 by Student's t test. Ctrl, control.