Figure 3.

Efficient gene deletion achieved following the treatment with CriPs targeting Gfp in primary GFP pre-adipocytes isolated from GFP mice. A and C, flow cytometry data of primary GFP pre-adipocytes treated with CriPs formulated in NEBuffer 3 (A) or PBS (C) (Cas9–sgRNA, 100 nm). B and D, different concentrations of EP with 100 nm of Cas9–sgRNA formulated in NEBuffer 3 (B) or PBS (D). E, percentage indel measurements by a T7E1 assay in genomic DNA isolated from primary GFP pre-adipocytes (uncut, 401 bp; cut, 288 bp + 113 bp). Error bars, S.E.