Figure 4.

Efficient gene deletion achieved following treatment with CriPs targeting Gfp in primary PECs isolated from thioglycollate-injected GFP mice. 5 days post-treatment, flow cytometry and T7E1 assay were performed to measure the loss of GFP. A and B, flow cytometry data of primary GFP PECs treated with CriPs with a control, nontargeting sgRNA sequence (Cas9–control sgRNA–EP) (A) and CriPs targeting Gfp (Cas9–Gfp sgRNA–EP) (B) (Cas9, 100 nm; sgRNA, 100 nm; EP, 2 μm). C, different concentrations of Cas9–sgRNA (1:1) with 2 μm EP. D, percentage indel measurements in Gfp genomic DNA isolated from CriPs-treated primary GFP PECs by a T7E1 assay (uncut, 401 bp; cut, 288 bp + 113 bp; Cas9, 100 nm; sgRNA, 100 nm; EP, 2 μm).