Figure 6.

Plasma cytokine levels were measured in mice intraperitoneally injected with CriPs in vivo. WT mice were intraperitoneally injected daily for 5 days with PBS or CriPs loaded with a nontargeting sgRNA. Plasma was collected before injection and then 24 h and 2 weeks after the first injection. Plasma cytokine levels (IL-1β, IL-4, IL-6, IL-10, IFNγ, and TNFα) were measured by Luminex. Plasma cytokine levels were measured before injection (A), 24 h after the first injection (B), and 2 weeks after the first injection (C). D, plasma cytokine levels (IL-10 and TNFα) were also measured in the mice treated with LPS at 1.5 h postinjection (p.i.) or without LPS to serve as positive controls for the assay. Data are means ± S.E. (error bars) (n = 3–4).