Table 1.

Size and charge measurements of particles

	Diameter	Polydispersity index	Charge
	nm		mV
EP	0.8 ± 0.2	0.272	18.0 ± 5.5
Cas9	9.3 ± 2.6	0.655	13.9 ± 6.8
Cas9–sgRNA (1:1)	14.3 ± 3.2	0.605	−2.4 ± 3.8
Cas9–sgRNA–EP (1:1:20)	375 ± 47	0.930	6.0 ± 4.4
Cas9–sgRNA–EP (1:1:150)	1098 ± 163	0.580	14.6 ± 6.3
Cas9–sgRNA–EP (1:1:250)	1285 ± 201	0.635	20.7 ± 6.0
Cas9–sgRNA–EP (1:1:20) in DMEM	347 ± 50	0.974	NAa
Cas9–sgRNA–EP (1:1:150) in DMEM	1270 ± 192	0.501	NA
Cas9–sgRNA–EP (1:1:250) in DMEM	1489 ± 283	0.387	NA

^a NA, not applicable.