Figure 2.
Nuclease activity of Exo1 and Dna2–Sgs1–RPA on circular dsDNA with a nick. A, reaction schematic. The asterisk denotes the 32P label in the substrate. B, the circular nick-containing substrate (1 nm) was incubated with Exo1 (0.25 nm) in the presence of RPA (800 nm) and/or MRX (16 nm) for the indicated times. The results from three independent experiments were graphed with the error bars representing S.D. C, the activity of Dna2 (8 nm) was tested with combinations of Sgs1 (8 nm), RPA (800 nm), TR (8 nm), and MRX (16 nm) as indicated. The results were graphed as in B. See also Fig. S2.