FIG 5.

Hypoxia-induced β-glucan masking is dependent on cAMP-PKA signaling. Cytometric analysis of β-glucan exposure on C. albicans cAMP-PKA mutants by Fc-dectin-1 staining of cells grown under normoxic (pink) or hypoxic conditions (cyan) (upper panels). Median fluorescence intensities (MFIs) for hypoxic and normoxic cell populations are shown. The corresponding wild-type control is shown above each mutant: WT, wild type (SN152) and cyr1Δ (CR323), tpk1Δ, tpk2Δ, and tpk1Δ tpk2Δ mutants (Table S1). The fold change in β-glucan exposure (lower panels) for each strain was calculated by dividing the MFI under hypoxic conditions by the MFI for the corresponding control normoxic cells. Means and standard deviations from three independent replicate experiments are shown, and the data were analyzed using ANOVA with Tukey’s multiple-comparison test: *, P ≤ 0.05; **, P ≤ 0.01.