FIG 8.

Exogenous dibutyryl-cAMP suppresses the defects in hypoxia-induced β-glucan masking caused by mitochondrial mutants. C. albicans (wild type [SC5314] [Table S1]), goa1Δ (GOA31), and upc2Δ cells (UPC2M4A) were grown under normoxic (pink) or hypoxic conditions (cyan) for 5 h, as described above, with 0 or 5 mM dibutyryl-cAMP (cAMP). The cells were then stained with Fc-dectin-1 and analyzed by flow cytometry to quantify their β-glucan exposure (upper panels). Median fluorescence intensities (MFIs) are shown. The fold changes in β-glucan exposure are shown (lower panels): means and standard deviations from three independent replicate experiments are analyzed using ANOVA with Tukey’s multiple-comparison test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.