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Differential gene expression and FK228 production. (A) RT-PCR profiling of Bth_depJ (representing Bth_dep gene cluster) expression in nine different media. Amplification of a 283-bp DNA from genomic template was included as a positive control. (B) HPLC profiling of FK228 productivity in M8 and M11 media with UV absorbance monitored at 235 nm. Insert shows an LC-MS chromatography of FK228 with [M + H]+ = 540.1 m/z.
