Fig. 5.

Treatment with MnP and dox reduces MDA-MB-231 cell invasion and extracellular proteolytic activity. MDA-MB-231 cells were seeded on matrigel-coated transwells and were allowed to invade for 16 h in the presence of the indicated drugs (dox (0.1 μM), MnP (5 μM)) (A). The percentage of invading cells is summarized in B. The extracellular proteolytic activity was measured using a fluorescent gelatine degradation assay (C). Invasion rates (B), and normalized gelatine degradation (D) from at least three independent experiments are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (Student's t-test, relative to untreated cells).