Fig. 7.

Effect of MnP and dox on the number of focal adhesions. Confocal images show MCF7 (A) and MDA-MB-231 (C) cells treated with the indicated drugs for 16 h, fixed and stained with anti pFAK. Images are typical of three independent experiments. Scale bars, 20 µm. Summary results (means ± SEM from ≥90 cells for each condition) show numbers of focal adhesions per cell, determined by counting pFAK positive spots (B and D). *p < 0.05, **p < 0.01 , ***p < 0.001 (one-way ANOVA with Tukey's test, relative to untreated cells).