Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 6;200(23):e00425-18. doi: 10.1128/JB.00425-18

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 American Society for Microbiology.

All Rights Reserved.

PMC Copyright notice

FIG 3 — Rod homologs are required for hook and filament assembly. Fluorescent micrographs of strains representing the presence of filament, hook, and basal body. Membranes were stained with FM-4-64 dye (false colored red). Filaments and hooks were visualized by using a maleimide-conjugated dye to stain proteins modified to introduce a single surface-exposed cysteine residue. (Hag^T209C and FlgE^T123C, respectively; false colored green). Basal bodies were visualized using a strain background containing a fluorescently tagged FliM-GFP (false colored green). The filament panel was generated with the following strains: DS1916 (WT), DK5040 (ΔflgB), DK5041 (ΔflgC) DS5897 (ΔflhO), DS5042 (ΔflhP), DK5038 (ΔfliF), DS5896 (ΔflgE), and DK5039 (ΔfliE). The hook panel was generated with the following strains: DS7673 (WT), DK4895 (ΔflgB), DK4868 (ΔflgC) DS8839 (ΔflhO), DS8840 (ΔflhP), DK3135 (ΔfliF), and DK4896 (ΔfliE). The basal body panel was generated with the following strains: DS8521 (WT), DK5082 (ΔflgB), DK5268 (ΔflgC) DK5083 (ΔflhO), DK5084 (ΔflhP), DS8598 (ΔfliF), DK4892 (ΔflgE), and DK5081 (ΔfliE). NP, not possible, as the cysteine replacement is within the gene that was deleted.