TABLE 1.
Strains used in this study
| Strain | Description | Reference(s) |
|---|---|---|
| E. coli | ||
| MFDpir | MG1655 RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecA Aprar Zeor Ermr | 50 |
| DH5α λpir | F− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λpir | 58 |
| S17-1 λpir | RP4-2(Km::Tn7 Tc::Mu-1) pro-82 λpir recA1 endA1 thiE1 hsdR17 creC510 | 59 |
| AP09 | E. coli WM5406/pHC001B | 49 |
| AP302 | E. coli DH5α/pSRK-Km | 51, 60 |
| AP1033 | MFDpir/pHC001B::crbSS337A/T338V | This study |
| AP834 | MFDpir/pHC001B::crbSΔtrans | This study |
| AP1244 | MFDpir/pSRK-Km::trans | This study |
| AP1576 | MFDpir/pHC001B::crbSΔSTAC | This study |
| AP1021 | E. coli/pACYC184 | 31 |
| AP1294a | TOP10/pRS415 | 31 |
| AP1212 | TOP10/pACYC184::crbR | This study |
| AP1221 | TOP10/pRS415::Pacs | This study |
| AP1228 | TOP10/pRS415::Pacs-small | This study |
| AP1337a | TOP10/pACYC184 and pRS415 | This study |
| AP1298a | TOP10/pACYC184 and pRS415::Pacs | This study |
| AP1323a | TOP10/pACYC184::crbR and pRS415::Pacs | This study |
| AP1324a | TOP10/pACYC184::crbR and pRS415::Pacs-small | This study |
| AP1207a | S17-1/pBBRlux::Pacs-P12 | This study |
| AP1211a | S17-1/pBBRlux::Pacs-P13 | This study |
| AP1039 | S17-1/pBBRlux::Pacs-P4 | This study |
| AP1047 | S17-1/pBBRlux::Pacs-P6 | This study |
| AP1194a | S17-1/pBBRlux::Pacs-P8 | This study |
| AP1197a | S17-1/pBBRlux::Pacs-P9 | This study |
| AP1043 | S17-1/pBBRlux::Pacs-P5 | This study |
| AP1200a | S17-1/pBBRlux::Pacs-P10 | This study |
| AP1203a | S17-1/pBBRlux::Pacs-P11 | This study |
| AP547 | MFDpir/pHC001B::Δcrp | This study |
| AP1970 | MFDpir/pHC001B::Δcya | This study |
| AP1968 | MFDpir/pHC001B::ΔrpoS | This study |
| AP1719 | S17-1/pBBRlux::PcrbS | This study |
| AP1715 | S17-1/pBBRlux::PcrbR | This study |
| AP915 | MFDpir/pSRKKm::crp | This study |
| AP1900 | MFDpir/pSRKKm::crbR | This study |
| AP211 | MFDpir/pHC001B::Δpta-ackA | This study |
| AP1934 | MFDpir/pHC001B::Δpta | This study |
| AP1932 | MFDpir/pHC001B::ΔackA | This study |
| V. cholerae | ||
| AP95 | V. cholerae SIO wild type | 48 |
| AP27 | SIO ΔcrbS | 21 |
| AP218 | SIO Δacs | 57 |
| AP1161 | crbSS337A/T338V | This study |
| AP462 | SIO/pBBRlux | 21 |
| AP431 | SIO/pPT002 | 21 |
| AP1659 | SIO crbSΔSTAC | This study |
| AP1693 | SIO crbSΔSTAC/pPT002 | This study |
| AP847 | SIO crbSΔtrans | This study |
| AP1288a | SIO crbSΔtrans::pSRK-Km | This study |
| AP1293a | SIO crbSΔtrans::pSRK-trans | This study |
| AP1986 | SIO Δacs/crbSΔSTAC | This study |
| AP1268 | SIO/pBBRlux::Pacs-P12 | This study |
| AP1242 | SIO/pBBRlux::Pacs-P13 | This study |
| AP1059 | SIO/pBBRlux::Pacs-P4 | This study |
| AP1075 | SIO/pBBRlux::Pacs-P6 | This study |
| AP1264 | SIO/pBBRlux::Pacs-P8 | This study |
| AP1234 | SIO/pBBRlux::Pacs-P9 | This study |
| AP1067 | SIO/pBBRlux::Pacs-P5 | This study |
| AP1236 | SIO/pBBRlux::Pacs-P10 | This study |
| AP1238 | SIO/pBBRlux::Pacs-P11 | This study |
| AP555 | SIO Δcrp | This study |
| AP1974 | SIO Δcya | This study |
| AP1979 | SIO ΔrpoS | This study |
| AP601 | SIO Δcrp/pPT002 | This study |
| AP1739 | SIO/pBBRlux::PcrbS | This study |
| AP1723 | SIO/pBBRlux::PcrbR | This study |
| AP1980 | SIO Δcrp/pBBRlux::PcrbS | This study |
| AP1982 | SIO Δcrp/pBBRlux::PcrbR | This study |
| AP1763 | SIO ΔcrbS/pBBRlux::PcrbS | This study |
| AP1755 | SIO ΔcrbR/pBBRlux::PcrbR | This study |
| AP683 | SIO/pSRK-Km | 57 |
| AP937 | SIO Δcrp/pSRK-Km::crp | This study |
| AP2002 | SIO Δcrp/pSRK-Km::crbR | This study |
| AP1996 | SIO ΔcrbR/pSRK-Km | This study |
| AP1906 | SIO ΔcrbR/pSRK-Km::crbR | This study |
| AP2000 | SIO ΔcrbR/pSRK-Km::crp | This study |
| AP229 | SIO Δpta-ackA | This study |
| AP1988 | SIO Δpta | This study |
| AP1992 | SIO ΔackA | This study |