FIG 12.

RNA repair operon expression in response to minocycline treatment. Cultures at mid-log phase were treated with 40 μg/ml minocycline (gray bars) or left untreated (black bars) and harvested after 2 h. Total RNA was extracted, and qRT-PCR was performed to assess rtcA transcript levels relative to the reference gene mdh. Each bar represents the average from 3 to 5 biological replicates; error bars represent ±1 standard deviation. Significant differences between treated and untreated or WT and ΔrtcR strains are denoted (*, P < 0.05; **, P < 0.1).