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. 2018 Nov;188(11):2653–2661. doi: 10.1016/j.ajpath.2018.07.012

Figure 1.

Figure 1

Generation of the transgenic mice with thyroid-specific expression of STRN-ALK fusion protein. A: Genetic construct containing sequences of the bovine thyroglobulin promoter (BTg Pro), reporter firefly luciferase–tandem dimmer Tomato (fLuc-tDt), and hemagglutinin (HA)-tagged STRN-ALK. Transcriptional activity of the promoter in thyroid cells was confirmed by detection of fLuc-tDt fluorescence and anti-HA immunoreactivity in immortalized normal thyroid HTori-3 cells transfected with initial plasmid containing the genetic construct. B: Immunofluorescence detection of the expression of the reporter tDt and anti-HA immunoreactivity in thyroid cells of founder Tg-STRN-ALK mouse. C and D: Western blot analysis and densitometry results showing expression of STRN-ALK in Tg-STRN-ALK mice thyroids (three to four thyroids per strain, age 6 months) and human STRN-ALK–positive papillary thyroid cancer sample. Original magnification: ×400 (A); ×200 (B). UTR, untranslated region.