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In vitro photobleaching rates of the conjugates at 5 μM solutions in 17% bovine calf serum (BCS) in phosphate buffer saline (PBS). HPPH-CD (8), HPPH2-CD (9), HPPH-Cypate (10) and HPPH2-Cypate (11) were in the order of 9 > 10 > 8 > 11. The rate of photobleaching of the chromophores was determined on the basis of reduced absorption at 661 nm (HPPH) and near 830 nm cyanine dye (CD) after irradiating with light (135 J/cm2, 75 mW/cm2) at 661 nm (in vitro absorption wavelength).
