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(A) Serum Ig anti-B. anthracis Sterne spores from mice immunized three times with different conjugated fragments, with and without Alum. (B–E) Competitive ELISA at different dilutions of α-tetrasaccharide, CRM197 and α-tetrasaccharide/CRM197 physically mixed with B. anthracis spores from Sterne (B), ΔbclA (C), ΔrmlD (D), or Δant1–4 (E).
