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. 2018 Aug 2;23(8):1932. doi: 10.3390/molecules23081932

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Development of the SCAR markers based on sequence variations in the nrDNA-ITS region. (A) Verification of primer specificities for C. militaris. (B) Verification of primer specificities for C. pruinosa. (C) Verification of primer specificities for I. cicadae. (D) Verification of primer specificities for I. tenuipes. (E) Verification of primer specificities for O. sinensis. The numbers 1–16 correspond to those listed in Table 1 in the “Gel lane” column. The precise lengths of the PCR products and DNA ladders are indicated to the right and left of the gel images, respectively. M, 100 bp DNA ladder.