Table 3.
Sequences of the sequence characterized amplified region (SCAR) primers, and the sizes and specificity of the amplified DNA fragments.
| Primer Name | Primer Sequence (5′→3′) | Amplicon Size (bp) | Species Specificity |
|---|---|---|---|
| CM F2 | GGCCCCAAACAGTGTATCTAC | 339 | C. militaris |
| CM R2 | CCGGTGCGAGTTGGCGTACTA | ||
| CM F3 * | CAACCCTTTGTGAACATACCT | 102 | |
| CM R3 * | GTAGATACACTGTTTGGGGCC | ||
| CP F2 | GACCCCAAACTCTGTTTCTAG | 244 | C. pruinosa |
| CP R2 | CCCCGCGAGGAGGGGTCGAGT | ||
| CP F1 * | ACTCGACCCCTCCTCGCGGGG | 116 | |
| CP R1 * | GTCCCGGTGCGACTGGTGTG | ||
| IC F1 | ACGCAACCCTGTATCCATCAG T | 337 | I. cicadae |
| IC R1 | TTCCCGGTGCGACTGGTTGT | ||
| IC F3 * | ACCCTTCTGTGAACCTACGCATC | 137 | |
| IC R3 * | GATTCAGCGAGACTGATGGAT | ||
| IT F4 * | CCTTCTGTGAACCTACCCATA | 132 | I. tenuipes |
| IT R3 * | GAGCGGCTCACAGATACAGG | ||
| IT F3 | CCATACTTGCTTCGGCGGACC | 107 | |
| IT R2 | GCTCACAGATACAGGGTTGC | ||
| OS F1 * | AGCGTCATCTCAACCCTCGAG | 200 | O. sinensis |
| OS R2 * | TGATCCGAGGTCAACTGGAGG | ||
| OS F3 | GAACACCACAGCAGTTGCCT | 117 | |
| OS R3 | GCTTCTTGACTGAGAGATGCC |
Asterisks (*) indicate SCAR markers used in the development of the real-time polymerase chain reaction (PCR) assay.