(a) Antibody profile of murine sera vaccinated with tetanus toxoid–glycopeptide conjugate when titrated against the Man3–BSA. All the sera were diluted 1:1000. The blue colored bars denote IgM response, whereas red colored bars represent IgG response. Under each category, solid bars represent final bleeds, whereas hashed bars denote first bleed. All the data points were plotted after subtracting the background value from similarly diluted pre-bleed sera. (b) Antibody profile of murine sera vaccinated with tetanus toxoid–glycopeptide conjugate when titrated against PVP–Fba. All the sera were diluted 1:1000. The blue colored bars denote IgM response, whereas red colored bars represent IgG response. Under each category, solid bars represent final bleed, whereas hashed bars denote first bleed. All the data points were plotted after subtracting the background value consisting of all assay reagents but sera.