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. 2018 Sep 20;23(10):2409. doi: 10.3390/molecules23102409

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of TPAE on H/R induced H9c2 cardiomyocytes apoptosis. H9c2 cells were pretreated with three dosages of TPAE for 24 h followed by H/R. (A) The apoptotic ratio of H9c2 cells was measured by flow cytometry using Annexin V-FITC and PI staining; (B) The apoptosis rate was quantified by BD FACSVerse software; (C) The relative mRNA expression of Bcl-2 and Bax was determined by qRT-PCR; (D) Immunoblots of Bcl-2, Bax and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was detected by western blotting; (E) Quantitative analysis of the ratio of Bax to Bcl-2 in protein expression was evaluated. Values were represented as mean ± SD (n = 3, each group). The results were representative of three independent experiments. ^# p < 0.05, ^## p < 0.05 vs. normal group; * p < 0.05, ** p < 0.01 vs. H/R group.