Figure 4.
COI1 is required for the synthesis of tobacco cembratriene-diols. (A,B) Trichome secretion droplets stained by I2/KI. (C) Soluble sugar content of the trichome secretion droplets. (D) Comparison of cembratriene-diol contents between the control (Ctrl) and plants with dysfunction of COI1 (COI1-RI). (E) The representative chromatogram profiles of cembratriene-diol determination by the UPLC system for the control and plants with dysfunction of COI1. (F) Transcription profiles of the cembranoid synthetic genes LTP1, CBTS and P450 in the control and plants with dysfunction of COI1. The transcription level of each gene in the control plants was arbitrarily set as “1”. Values in (C,D,F) are the average of data from three independent lines with triplicate measurements. Asterisks in (C,D,F) indicate significant difference from the control plants. The inset in (E) shows the enlargement of the chromatogram image in the blue rectangle, and α and β in the graph of (E) indicate α-cembratriene-diol and β-cembratriene-diol, respectively. Error bars, mean ± SE.