Figure 5.
Regulatory roles of NtMYC2a in COI1-mediated cembratriene-diol formation in tobacco. (A,B) Trichome secretion droplets staining with I2/KI for plants with dysfunction of COI1 (COI1-RI) and overexpressing NtMYC2a in plants with dysfunction of COI1 (COI1-RI/MYC2-OE). (C) Soluble sugar content of the trichome secretion droplets in the plants of COI1-RI and COI1-RI/MYC2-OE. (D) Comparison of cembratriene-diol contents between the plants of COI1-RI and COI1-RI/MYC2-OE. (E) The representative chromatogram profiles of cembratriene-diol determination by the UPLC system for the plants of COI1-RI and COI1-RI/MYC2-OE. The triangle indicates the unidentified compound observed in above results. (F) Transcription profiles of the cembranoid synthetic genes LTP1, CBTS and P450 in the plant set of the control (Ctrl), COI1-RI and COI1-RI/MYC2-OE. The transcription level of each gene in the control (Ctrl) plants was arbitrarily set as “1”. Values in (C,D,F) are the average of data from three independent lines with triplicate measurements. Asterisks in (C,D,F) indicate significant difference from the plants of COI1-RI. Error bars, mean ± SE.