Figure 5.

LDH activity induced by quercetin, quercetagetin and patuletin on CaSki, MDA-MB-231 and SK-Lu-1 cultures. In each case, 7500 cells/well were seeded in 96-well tissue culture plates. After 24 h, the medium was removed, and the cells were stimulated with the IC₅₀ values for quercetin, quercetagetin and patuletin, or EtOH (10 μL/mL). The amount of lactate dehydrogenase (LDH) released into the culture supernatant was measured after 24 h of treatment. Experimental data are presented as mean ± S.D. for three independent experiments with three repetitions. * p < 0.05 versus EtOH (ANOVA followed by a Tukey’s test).