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The anti-EV71 effect of compound 2d in Vero cells with BBR as a positive control. (A) 2d reduced the EV71-induced CPE. Cells were examined using a microscopy (×40); (B) 2d reduced the EV71-induced CPE. Cells were examined using crystal violet staining; (C) 2d reduced the expression of EV71 VP1 RNA by one-step qRT-PCR assay. ** p < 0.001 * p < 0.05; (D) 2d reduced the expression of EV71 VP1 protein by western blot assay.
