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Compound 2d inhibits the phosphorylation of MEK/ERK with BBR as a control. Vero cells (9 × 105 cells/well) were plated into 6-well culture plates and were mock-infected or infected with EV71 (H, MOI = 0.1) for 1 h. The cells were then treated with compound 2d for 24 h and were harvested for western blot assay.
