Figure 1.

Deletion of Zip1 causes reduced spore viability and increased dyads. (A) Spore viability assay in wild-type (SGY40), zip1Δ (SGY1066), and mam1Δ (SGY223). (B) Sporulation efficiency was determined by counting 200 cells for each strain in two independent experiments. (C) Field view of the sporulation cultures of the above strains after 24 h in SPM medium. Blue, and red arrowheads show tetrad/triad and dyads, respectively. Bar, ∼5 µm. (D) Distribution of types of asci in the above strains. Sporulation efficiency and type of asci were calculated after repetition of the experiments two times. (E) The percentage of tetrad type represents tetrads with 4, 3, 2, 1 and 0 spores viable (4-sv, 3-sv, 2-sv, 1-sv and 0-sv). % tetrad type was calculated from total 60 tetrads dissected for each strain. The spore viability and the percentage of tetrad type were calculated after tetrad dissection for two times (30 dissected in each time). Error bars represent the standard deviation from the mean.