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. 2018 Sep 25;8(11):3691–3701. doi: 10.1534/g3.118.200469

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Copyright © 2018 by the Genetics Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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zip1Δ mutant shows chromosome segregation defects similar to mam1Δ. (A) The histograms show the percentage of asymmetric and symmetric tetrads in wild-type (SGY116), mam1Δ (SGY223) and zip1Δ (SGY1127) strains. (B) Segregation pattern of homozygous GFP dots in the above strains. (C) Segregation pattern of homozygous GFP dots in the dyads of mam1Δ and zip1Δ strains. (D) Immunofluorescence of the above strains showing the percentage of anaphase I cells harboring mono- or bi-nucleates. DNA and spindle were visualized using DAPI and anti-tubulin antibodies, respectively. The absence of Zip1 gives an equal number of mono-nucleates like mam1Δ at the end of meiosis I. Bar, ∼2 µm. Two independent experiments were performed for each strain, and the average values are shown with a total number of cells represented by N. Error bars represent the standard deviation from the mean.