Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 13;8(11):3593–3605. doi: 10.1534/g3.118.200539

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Huynh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Somatic CRISPR in the Drosophila prothoracic gland. In Drosophila larvae, the prothoracic gland (PG) is the principal source for ecdysteroid production. The PG is a part of the ring gland, which also harbors the corpora allata (yellow) and corpora cardiaca (green). PG-specific genome editing via Clustered Regularly Interspaced Short Palidromic Repeats (CRISPR) requires the recruitment of CRISPR-associated protein 9 (Cas9: green) to the target site recognized by the guide RNA (gRNA: orange). Target site cleavage by Cas9 is ensured by the presence of the protospacer adjacent motif (PAM: purple) sequence immediately following the target site. This sequence will direct the cut site of Cas9 to a region of about three nucleotides upstream of the PAM.