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Nuclear localization of Cas9 in the Drosophila prothoracic gland (PG). Nuclear localization sequences were added to the 5′ and 3′ ends of the Cas9 cDNA to ensure transport of Cas9 into nuclei. The spok regulatory region drives the expression of Cas9 specifically in PG cells with no detectable signal in the adjacent corpora allata and the corpora cardiaca. DAPI (blue) was used to stain DNA while anti-Cas9 antibodies (red) was used to detect Cas9.
