Figure 6.

Efficiency of tissue-specific CRISPR/CAS9 in the Drosophila prothoracic gland (PG). A. Comparing phenotypes of a classic disembodied mutant (dib²) and PG-specific RNAi (dib^IR) with PG-specific (spok_DmC, spok_HsC and spok_dFC) or ubiquitous CRISPR/Cas9 (act-Cas9.P2). B. Comparing phenotypes of a classic phantom mutant (phm^E7) and PG-specific RNAi (phm^IR) with PG-specific (spok_DmC, spok_HsC and spok_dFC) or ubiquitous CRISPR/Cas9 (act-Cas9.P2) in combination with dU6-dib^gR1 or dU6-phm^gR2. C and D. Sequences of dib (C) and phm (D) loci from brain and PG nuclei, using either spok_DmC or spok_HsC in combination with dU6-dib^gR1 or dU6-phm^gR1. E. Sequences of dib locus from brain and PG nuclei using spok_dFC.