Figure 1.
a) Polymer anchors with carboxy-functionality surrounded by a supported lipid bilayer spiked with a biotin-modified lipid (pink). b) NHS groups covalently bound to the carboxy groups. c) NH2–NTA bound to the NHS-functional polymer structure. d) Albumin (light gray circles) blocks the remaining carboxy groups and NHS esters. This step prevents unspecific protein binding to the polymer anchors. Subsequently, the NTA head groups are chelated with nickel ions. e) Immobilization of histidine-tagged fluorescent proteins. f) Streptavidin-Alexa Fluor 555 was adhered to the biotinylated lipid bilayer. All schemes are not to scale.