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. 2018 Oct 16;90(21):12372–12376. doi: 10.1021/acs.analchem.8b02588

Figure 2.

Figure 2

Specific anchoring of histidine-tagged proteins to Ni-NTA modified acrylate structures. a) SEM image of a nonfunctional grid (orange dashed lines) superimposed with a carboxy-functional one (blue dashed lines), offset by half a period in x and y directions. b) A fluorescence microscopy image of the same structure shows strong protein (EmGFP) binding to the Ni-NTA-functional structure but only weak binding to the pure PETA grid. Excitation wavelength 491 nm. The scale bar of 5 μm in (b) is valid for (a) and (b).