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. 2018 Oct 10;23(10):2585. doi: 10.3390/molecules23102585

Figure 2.

Figure 2

The characterization of the luciferase reporter gene assay system. (A) The dose-dependent inhibitory effects of haloperidol against dopamine D1 receptor. Data were analyzed with Prism 5 using nonlinear regression. The results are expressed as the mean ± SD (n = 3); (B) The calculation of the Z′-factor in a 96-well plate format of this assay. Cells were treated with haloperidol (2 μM) or vehicle for 1 h, then incubated with dopamine (2 nM) for another 6 h.