. 2018 Oct 12;23(10):2620. doi: 10.3390/molecules23102620

Table 5.

List of primers, T_M, and extension time.

Genomic Regions	Sequence in 5′-3′	Source	T_M	Extension Time at 72 °C
ETS1f	CTTTTTGTGCATAATGTATATATAGGGGG	Linder et al. [58]	45 °C	60 s
18S-2L	TGACTACTGGCAGGATCAACCAG	Linder et al. [58]	45 °C	60 s
ITS4	TCCTCCGCTTATTGATATGC	White et al. [59]	52 °C	30 s
ITS5	GGAAGTAAAAGTCGTAACAAGG	White et al. [59]	52 °C	30 s
trnL-F-trnC	CGAAATCGGTAGACGCTACG	Taberlet et al. [60]	52 °C	60 s
trnL-F-trnF	ATTTGAACTGGTGACACGAG	Taberlet et al. [60]	52 °C	60 s
psbA	CGAAGCTCCATCTACAAATGG	Hamilton et al. [61]	56 °C	30 s
trnH (GUG)	ACTGCCTTGATCCACTTGGC	Hamilton et al. [61]	56 °C	30 s

T_M = Melting temperature.