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. 2018 Oct 16;23(10):2659. doi: 10.3390/molecules23102659

Figure 3.

Figure 3

Purification of recombinant OsCADs expressed in E. coli. The His-tagged OsCAD1, 2, 6 and 7 were expressed in E. coli in a soluble form. The recombinant proteins were purified by Ni2+-affinity chromatography. (A) Representative purification of the recombinant OsCAD2 protein. (B) Affinity purified recombinant OsCAD proteins. M, Molecular weight marker.