Table 4.
Derived kinetic constants for zidovudine glucuronidation in human (HLM) and rat liver microsomes (RLM) obtained from the incubation with and without 1% (w/v) bovine serum albumin (BSA). Data are presented as mean ± SD.
| Without 1% BSA | With 1% BSA | |||||
|---|---|---|---|---|---|---|
| Vmax (pmol/mg/min) | Km (mM) | CLint (µL/mg/min) | Vmax (pmol/mg/min) | Km (mM) | CLint (µL/mg/min) | |
| HLM | 1092 ± 19.10 | 3.40 ± 0.70 | 0.32 | 1450 ± 26.05 | 0.88 ± 0.05 | 1.65 |
| RLM | 770 ± 39.82 | 8.84 ± 1.19 | 0.09 | 1089 ± 33.50 | 6.77 ± 0.60 | 0.16 |
Vmax = maximal velocity; Km = Michaelis constant; CLint = intrinsic clearance.