. 2018 Oct 16;23(10):2650. doi: 10.3390/molecules23102650

Table 2.

Performance of the method determined during validation.

Method	Matrix	Spike (g/100 g)		Measured (g/100 g)		Recovery (%)		RSD(r) (%)		RSD (iR) (%)
Method	Matrix	2′-FL	LNnT	2′-FL	LNnT	2′-FL	LNnT	2′-FL	LNnT	2′-FL	LNnT
HPAEC-PAD	IF powder with intact protein	0.590	0.204	0.583	0.226	98.9	111	2.7	3.5	4.2	7.2
		0.751	0.357	0.735	0.367	97.8	103	1.7	1.1	2.2	2.1
		0.921	0.510	0.886	0.501	96.2	98.1	0.47	1.2	2.2	3.2
HPAEC-PAD	IF powder with partially hydrolyzed protein	0.590	0.204	0.573	0.222	97.0	109	5.2	5.6	4.3	7.9
		0.751	0.357	0.710	0.363	94.6	102	4.8	4.9	5.7	5.6
		0.921	0.510	0.870	0.499	94.4	97.9	1.6	1.7	2.4	3.7
HILIC-FLD	IF powder with intact protein	0.571	0.194	0.557	0.191	97.5	98.8	1.2	2.1	2.7	2.7
		0.723	0.338	0.702	0.331	97.1	97.9	1.7	1.9	3.3	3.2
		0.880	0.484	0.857	0.473	97.4	97.9	1.9	2.3	2.8	3.1
HILIC-FLD	IF powder with partially hydrolyzed protein	0.822	0.425	0.805	0.397	97.9	93.5	1.7	1.2	2.7	4.4
HILIC-FLD	IF powder with intact protein and FOS	0.573	0.193	0.570	0.188	99.6	97.4	1.8	2.4	2.7	4.7
		0.722	0.384	0.710	0.379	98.3	98.5	1.6	1.5	3.4	2.1
		0.874	0.582	0.855	0.579	97.9	99.5	1.2	1.5	2.6	2.5
HILIC-FLD	FUF powder with intact protein and FOS	0.575	0.191	0.557	0.189	96.8	99.1	2.1	1.8	7.4	4.9
		0.722	0.387	0.695	0.390	96.3	101	2.0	1.6	4.5	2.6
		0.877	0.567	0.824	0.576	93.9	102	2.2	1.4	4.2	2.3
HILIC-FLD	IF RTF with intact protein and FOS	0.541 ^a	0.222 ^a	0.548 ^a	0.224 ^a	101	101	1.5	2.3	2.6	3.5
		1.06 ^a	0.546 ^a	1.06 ^a	0.568 ^a	100	104	1.8	2.0	2.0	2.5
		4.17 ^a	1.05 ^a	4.19 ^a	1.09 ^a	101	104	1.2	2.6	2.2	4.8
HILIC-FLD	IF powder with intact protein and FOS (pilot production)	n/a	n/a	0.785	0.390	n/a	n/a	1.5	2.2	3.3	5.0
HILIC-FLD	FUF powder with intact protein and FOS (pilot production)	n/a	n/a	0.600	0.304	n/a	n/a	1.3	1.8	5.0	3.1

^a Values in g/L. Abbreviations: FOS = fructooligosaccharides, FUF = follow-up-formula, IF = Infant formula, RTF = ready to feed.